Immunocytochemistry-Immunofluorescence analysis of PDXDC1 in U2OS cells. Cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with PDXDC1 Polyclonal Antibody (Product # PA5-112232) (1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to Golgi apparatus.